concentration-response curves of four-parameter non-linear regression analysis Search Results


four-parameter non-linear regression algorithm  (CambridgeSoft Corporation)
90
CambridgeSoft Corporation four-parameter non-linear regression algorithm
Four Parameter Non Linear Regression Algorithm, supplied by CambridgeSoft Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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originpro 9.0  (OriginLab corp)
90
OriginLab corp originpro 9.0
Originpro 9.0, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/concentration-response+curves+of+four-parameter+non-linear+regression+analysis/originpro+software/pm31796277-135-33-35
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originpro 9.0 - by Bioz Stars, 2026-10
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four parameter variable slope model  (GraphPad Software Inc)
99
GraphPad Software Inc four parameter variable slope model
Four Parameter Variable Slope Model, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/concentration-response+curves+of+four-parameter+non-linear+regression+analysis/Prism+8/10__1016_slash_j__ejmcr__2026__100374-217-11-18
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four parameter variable slope model - by Bioz Stars, 2026-10
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bio-lite luciferase assay system  (Vazyme Biotech Co)
96
Vazyme Biotech Co bio-lite luciferase assay system
Bio Lite Luciferase Assay System, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/concentration-response+curves+of+four-parameter+non-linear+regression+analysis/Bio-Lite+Luciferase+Assay+System/custom%40dd1201%4010950932
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parameter dose response model  (GraphPad Software Inc)
99
GraphPad Software Inc parameter dose response model
Parameter Dose Response Model, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/concentration-response+curves+of+four-parameter+non-linear+regression+analysis/Prism+6/10__1186_slash_s12917___026___05859___0-53-46-51
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parameter dose response model - by Bioz Stars, 2026-10
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e64 d  (Gilead Sciences)
99
Gilead Sciences e64 d
Inhibitory effect of <t>E64-D</t> and remdesivir on a SARS-CoV-2-GFP replicon. A , B 293 T cells were transfected with SARS-CoV-2-GFP replicon, and ten hours post-transfection, cells were treated or nontreated (BLANK) with E64-D at indicated concentrations. Thirty-six hours post-transfection, cells were observed and photographed by fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( A ) or lysed for Western blot monitored the expression of tGFP-BlaR, actin was used as a loading control ( B ). C , D 293 T cells were transfected SARS-CoV-2-GFP replicon, and cells were treated or nontreated (BLANK) with remdesivir at indicated concentrations just before transfection. Thirty-six hours post-transfection, cells were observed and photographed using fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( C ), or lysed for Western blot for tGFP-BlaR, Actin was used as a loading control ( D ).
E64 D, supplied by Gilead Sciences, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/concentration-response+curves+of+four-parameter+non-linear+regression+analysis/VEKLURY/pmc08034055-139-4-9
Average 99 stars, based on 1 article reviews
e64 d - by Bioz Stars, 2026-10
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sigmaplot  (SYSTAT)
99
SYSTAT sigmaplot
Inhibitory effect of <t>E64-D</t> and remdesivir on a SARS-CoV-2-GFP replicon. A , B 293 T cells were transfected with SARS-CoV-2-GFP replicon, and ten hours post-transfection, cells were treated or nontreated (BLANK) with E64-D at indicated concentrations. Thirty-six hours post-transfection, cells were observed and photographed by fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( A ) or lysed for Western blot monitored the expression of tGFP-BlaR, actin was used as a loading control ( B ). C , D 293 T cells were transfected SARS-CoV-2-GFP replicon, and cells were treated or nontreated (BLANK) with remdesivir at indicated concentrations just before transfection. Thirty-six hours post-transfection, cells were observed and photographed using fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( C ), or lysed for Western blot for tGFP-BlaR, Actin was used as a loading control ( D ).
Sigmaplot, supplied by SYSTAT, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/concentration-response+curves+of+four-parameter+non-linear+regression+analysis/SigmaPlot+v14%2E0/pmc07475246-54-43-45
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pcr plates  (Bio-Rad)
99
Bio-Rad pcr plates
Inhibitory effect of <t>E64-D</t> and remdesivir on a SARS-CoV-2-GFP replicon. A , B 293 T cells were transfected with SARS-CoV-2-GFP replicon, and ten hours post-transfection, cells were treated or nontreated (BLANK) with E64-D at indicated concentrations. Thirty-six hours post-transfection, cells were observed and photographed by fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( A ) or lysed for Western blot monitored the expression of tGFP-BlaR, actin was used as a loading control ( B ). C , D 293 T cells were transfected SARS-CoV-2-GFP replicon, and cells were treated or nontreated (BLANK) with remdesivir at indicated concentrations just before transfection. Thirty-six hours post-transfection, cells were observed and photographed using fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( C ), or lysed for Western blot for tGFP-BlaR, Actin was used as a loading control ( D ).
Pcr Plates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/concentration-response+curves+of+four-parameter+non-linear+regression+analysis/Hard-Shell+96-Well+PCR+Plates/pm37149038-69-47-50
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pcr plates - by Bioz Stars, 2026-10
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Inhibitory effect of E64-D and remdesivir on a SARS-CoV-2-GFP replicon. A , B 293 T cells were transfected with SARS-CoV-2-GFP replicon, and ten hours post-transfection, cells were treated or nontreated (BLANK) with E64-D at indicated concentrations. Thirty-six hours post-transfection, cells were observed and photographed by fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( A ) or lysed for Western blot monitored the expression of tGFP-BlaR, actin was used as a loading control ( B ). C , D 293 T cells were transfected SARS-CoV-2-GFP replicon, and cells were treated or nontreated (BLANK) with remdesivir at indicated concentrations just before transfection. Thirty-six hours post-transfection, cells were observed and photographed using fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( C ), or lysed for Western blot for tGFP-BlaR, Actin was used as a loading control ( D ).

Journal: Virologica Sinica

Article Title: Construction of Non-infectious SARS-CoV-2 Replicons and Their Application in Drug Evaluation

doi: 10.1007/s12250-021-00369-9

Figure Lengend Snippet: Inhibitory effect of E64-D and remdesivir on a SARS-CoV-2-GFP replicon. A , B 293 T cells were transfected with SARS-CoV-2-GFP replicon, and ten hours post-transfection, cells were treated or nontreated (BLANK) with E64-D at indicated concentrations. Thirty-six hours post-transfection, cells were observed and photographed by fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( A ) or lysed for Western blot monitored the expression of tGFP-BlaR, actin was used as a loading control ( B ). C , D 293 T cells were transfected SARS-CoV-2-GFP replicon, and cells were treated or nontreated (BLANK) with remdesivir at indicated concentrations just before transfection. Thirty-six hours post-transfection, cells were observed and photographed using fluorescence microscopy, the nuclei were stained with Hoechst, scale bar: 100 µm ( C ), or lysed for Western blot for tGFP-BlaR, Actin was used as a loading control ( D ).

Article Snippet: EC 50 value of E64-D ( F ) and remdesivir ( G ) as determined by dose response curves using the ratio of tGFP-BlaR/β-actin gray values with different concentration and calculated by four-parameter nonlinear regression.

Techniques: Transfection, Fluorescence, Microscopy, Staining, Western Blot, Expressing

Determining the EC 50 of E64-D and remdesivir using a SARS-CoV-2-GFP replicon. A , B 293 T cells were transfected with SARS-CoV-2-GFP replicon, E64-D and remdesivir were added to culture media 10 h post transfection or just before transfection respectively, the green fluorescence intensity was determined thirty-six hours post-transfection, and dose responsive curves were drawn and EC 50 value of E64-D ( A ) and remdesivir ( B ) was calculated by four-parameter nonlinear regression respectively. Cell viability was determined by CCK8 assay in parallel on 293 T cells. The experiments were done in triplicates. C – F Replicon transfection and drug treatment were performed as mentioned in ( A , B ), thirty-six hours post-transfection, Western blot for tGFP-BlaR expression was carried out with E64-D ( C ) and remdesivir treated cells ( E ). EC 50 value of E64-D ( F ) and remdesivir ( G ) as determined by dose response curves using the ratio of tGFP-BlaR/β-actin gray values with different concentration and calculated by four-parameter nonlinear regression. The experiments were done at least two times.

Journal: Virologica Sinica

Article Title: Construction of Non-infectious SARS-CoV-2 Replicons and Their Application in Drug Evaluation

doi: 10.1007/s12250-021-00369-9

Figure Lengend Snippet: Determining the EC 50 of E64-D and remdesivir using a SARS-CoV-2-GFP replicon. A , B 293 T cells were transfected with SARS-CoV-2-GFP replicon, E64-D and remdesivir were added to culture media 10 h post transfection or just before transfection respectively, the green fluorescence intensity was determined thirty-six hours post-transfection, and dose responsive curves were drawn and EC 50 value of E64-D ( A ) and remdesivir ( B ) was calculated by four-parameter nonlinear regression respectively. Cell viability was determined by CCK8 assay in parallel on 293 T cells. The experiments were done in triplicates. C – F Replicon transfection and drug treatment were performed as mentioned in ( A , B ), thirty-six hours post-transfection, Western blot for tGFP-BlaR expression was carried out with E64-D ( C ) and remdesivir treated cells ( E ). EC 50 value of E64-D ( F ) and remdesivir ( G ) as determined by dose response curves using the ratio of tGFP-BlaR/β-actin gray values with different concentration and calculated by four-parameter nonlinear regression. The experiments were done at least two times.

Article Snippet: EC 50 value of E64-D ( F ) and remdesivir ( G ) as determined by dose response curves using the ratio of tGFP-BlaR/β-actin gray values with different concentration and calculated by four-parameter nonlinear regression.

Techniques: Transfection, Fluorescence, CCK-8 Assay, Western Blot, Expressing, Concentration Assay

Construction a SARS-CoV-2-GFP-FLuc replicon and determination of the EC 50 of E64-D. A Diagram of SARS-CoV-2-GFP-FLuc replicon. B Verification of a SARS-CoV-2-GFP-FLuc replicon plasmid. Recombinant SARS-CoV-2-GFP-FLuc replicon plasmid was tested for all sub-genomic fragments used in yeast homologous recombination by PCR. C Luciferase activity in nontransfected (BLANK) or SARS-CoV-2-GFP-FLuc replicon-transfected or 293 T cells, the luciferase activity was determined 36 h post transfection. D Kinetics study of luciferase activity after SARS-CoV-2-GFP-FLuc replicon transfection, 293 T cells were transfected with SARS-CoV-2-GFP-Fluc replicon and luciferase activity was determined at indicated hours post-transfection (hpt). E EC 50 value of E-64D was determined using a dose response curve drawn based on luciferase activity in SARS-CoV-2-GFP-FLuc-transfected 293 T cells using four-parameter nonlinear regression. F EC 50 value of E-64D was determined using a dose response curve drawn based on the ratio of tGFP-BlaR/β-actin gray values with different concentration and calculated by four-parameter nonlinear regression, the experiments were done at least two times.

Journal: Virologica Sinica

Article Title: Construction of Non-infectious SARS-CoV-2 Replicons and Their Application in Drug Evaluation

doi: 10.1007/s12250-021-00369-9

Figure Lengend Snippet: Construction a SARS-CoV-2-GFP-FLuc replicon and determination of the EC 50 of E64-D. A Diagram of SARS-CoV-2-GFP-FLuc replicon. B Verification of a SARS-CoV-2-GFP-FLuc replicon plasmid. Recombinant SARS-CoV-2-GFP-FLuc replicon plasmid was tested for all sub-genomic fragments used in yeast homologous recombination by PCR. C Luciferase activity in nontransfected (BLANK) or SARS-CoV-2-GFP-FLuc replicon-transfected or 293 T cells, the luciferase activity was determined 36 h post transfection. D Kinetics study of luciferase activity after SARS-CoV-2-GFP-FLuc replicon transfection, 293 T cells were transfected with SARS-CoV-2-GFP-Fluc replicon and luciferase activity was determined at indicated hours post-transfection (hpt). E EC 50 value of E-64D was determined using a dose response curve drawn based on luciferase activity in SARS-CoV-2-GFP-FLuc-transfected 293 T cells using four-parameter nonlinear regression. F EC 50 value of E-64D was determined using a dose response curve drawn based on the ratio of tGFP-BlaR/β-actin gray values with different concentration and calculated by four-parameter nonlinear regression, the experiments were done at least two times.

Article Snippet: EC 50 value of E64-D ( F ) and remdesivir ( G ) as determined by dose response curves using the ratio of tGFP-BlaR/β-actin gray values with different concentration and calculated by four-parameter nonlinear regression.

Techniques: Plasmid Preparation, Recombinant, Homologous Recombination, Luciferase, Activity Assay, Transfection, Concentration Assay